- We have developed efficient protocols for the phytochemical analyses of natural compounds with biological activities.
- Phytochemical analyses of plant extracts and commercial natural products.
- Testing of the biological activity of plant extracts and commercial natural products.
- In vitro plants synthesized and stored significant quantities of natural compounds and represent a promising system for obtaining qualitatively and quantitatively standardized extracts for the pharmaceutical and cosmetic industries.
Phytochemical Analyses of Commercial Natural Plant Products
| Chromatographic Analyses of Bioactive Compounds (HPLC/DAD/ESI-MSn and UPLC-TUV) |
| Acyl-phloroglucinols |
| Anthocyanins |
| Cannabinoids (Ph. Eur.) |
| Flavanols |
| Flavonoid aglycones |
| Flavonol glycosides |
| Naphtodianthrones/Hypericins (Ph. Eur.) |
| Phenolic acids |
| Xanthones |
| Spectrophotometric Analyses of Bioactive Compounds |
| Anthocyanins (pH differential method) |
| Cannabinoids (Ph. Eur.) |
| Catechins/Flavanols (DMACA reagent) |
| Flavonoids (AlCl3 reagent) |
| Hypericins (Ph. Eur.) |
| Phenolic acids (Arnow reagent) |
| Phenolic compounds (Folin-Ciocalteu reagent) |
| Proanthocyanidins (Butanol-HCl reagent) |
| Non-Enzymatic Antioxidants |
| Ascorbic acid (DTT reagent) |
| Carotenoids (Acetone reagent) |
| Chlorophylls (Acetone reagent) |
| Glutathione (DTNB reagent) |
| Tocopherols (α-α’-bipyridyl and FeCl3 reagents) |
| Antioxidant Enzymes |
| Ascorbate peroxidase activity (APX, EC 1.11.1.11) |
| Catalase activity (CAT, EC 1.11.1.6) |
| Glutathione reductase activity (GR, EC 1.6.4.2) |
| Guaiacol peroxidase activity (PX, EC 1.11.1.7) |
| Superoxide dismutase activity (SOD, EC 1.15.1.1) |
| Antioxidant Activity |
| ABTS radical scavenging activity |
| Cupric ion reducing antioxidant capacity (CUPRAC assay) |
| DPPH radical scavenging activity |
| Ferric ion reducing antioxidant power (FRAP assay) |
| Ferrous chelating activity (FCA assay) |
| Lipid peroxidation inhibition (LPI assay) |
| Phosphomolybdenum (PM) assay |
| Reducing power (RP assay) |
| Reactive Oxygen/Nitrogen Species (ROS/RNS) Scavenging Activity |
| Hydrogen peroxide (H2O2) scavenging activity |
| Hydroxyl radical (OH•) scavenging activity |
| Nitric oxide (NO) scavenging activity |
| Superoxide radicals (O2•-) scavenging activity |
| Oxidative Stress Marker Contents |
| Hydrogen peroxide (H2O2) production |
| Superoxide radical (O2•-) production |
| Malondialdehyde (MDA) content |
Biological Activity of Commercial Natural Plant Products
| Skincare and Anti-Aging Products |
| UV-protective activity (UV-spectra method) |
| Skin Depigmentation (Inhibition of Tyrosinase activity) |
| Anti-aging and anti-wrinkle activity (Inhibition of Elastase activity) |
| Anti-aging and anti-wrinkle activity (Inhibition of Collagenase activity) |
| Antidepressant Products |
| Antidepressant activity (Inhibition of Monoamine oxidase A activity) |
| Neuroprotective Products |
| Neuroprotective activity (Inhibition of Acetylcholinesterase activity) |
| Neuroprotective activity (Inhibition of Butyrylcholinesterase activity) |
| Anti-Inflammatory Products |
| Anti-inflammatory activity (Inhibition of β-glucuronidase activity) |
| Anti-inflammatory activity (Inhibition of Lipoxygenase activity) |
| Anti-inflammatory activity (Inhibition of Xanthine oxidase activity) |
| Antidiabetic Products |
| Antidiabetic activity (Inhibition of α-amylase activity) |
| Antidiabetic activity (Inhibition of α-glucosidase activity) |
| Antihyperlipidemic Products |
| Antihyperlipidemic activity (Inhibition of Pancreatic lipase activity) |
| Antihyperlipidemic activity (Inhibition of Cholesterol esterase activity) |
| Gastroprotective Products |
| Gastroprotective activity (Inhibition of Urease activity) |
| Cardioprotective Products |
| Cardioprotective activity (Inhibition of Phosphodiesterase activity) |
| Antitoxic Products |
| Antitoxic activity (Inhibition of Carboxyl esterase activity) |
| Antiprotease Products |
| Antiprotease activity (Inhibition of α-chymotrypsin) |
| Antiprotease activity (Inhibition of trypsin) |
| In Vitro Digestion of Natural Products (Bioaccessibility and Bioavailability) |
| In vitro oral digestion (Simulation of salivary digestion) |
| In vitro gastric digestion (Simulation of gastric digestion) |
| In vitro intestinal digestion (Simulation of intestinal digestion) |