PHYTOCHEMICAL ANALYSES OF COMMERCIAL
NATURAL PLANT PRODUCTS
| CHROMATOGRAPHIC ANALYSES OF BIOACTIVE COMPOUNDS (HPLC/DAD/ESI-MSn and UPLC-TUV) |
| Acyl-phloroglucinols |
| Anthocyanins |
| Cannabinoids (Ph. Eur.) |
| Flavanols |
| Flavonoid aglycones |
| Flavonol glycosides |
| Naphtodianthrones/Hypericins (Ph. Eur.) |
| Phenolic acids |
| Xanthones |
| SPECTROPHOTOMETRIC ANALYSES OF BIOACTIVE COMPOUNDS |
| Anthocyanins (pH differential method) |
| Cannabinoids (Ph. Eur.) |
| Catechins/Flavanols (4-(Dimethylamino) cinnamaldehyde – DMACA reagent) |
| Flavonoids (AlCl3 reagent) |
| Hypericins (Ph. Eur.) |
| Phenolic acids (Arnow regent) |
| Phenolic compounds (Folin-Ciocalteu reagent) |
| Proanthocyanidins (Butanol-HCl reagent) |
| NON-ENZYMATIC ANTIOXIDANTS |
| Ascorbic acid (Dithiothreitol – DTT reagent) |
| Carotenoids (Acetone reagent) |
| Chlorophylls (Acetone reagent) |
| Glutathione (5,5′-dithiobis(2-nitrobenzoic acid – DTNB reagent) |
| Tocopherols (α-α’-bipyridyl and FeCl3 reagents) |
| ANTIOXIDANT ENZYMES |
| Ascorbate peroxidase activity (APX, EC 1.11.1.11) |
| Catalase activity (CAT, EC 1.11.1.6) |
| Glutathione reductase activity (GR, EC 1.6.4.2) |
| Guaiacol peroxidase activity (PX, EC 1.11.1.7) |
| Superoxide dismutase activity (SOD, EC 1.15.1.1) |
| ANTIOXIDANT ACTIVITY |
| ABTS [2.2’-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt] radical scavenging activity |
| Cupric ion reducing antioxidant capacity (CUPRAC assay) |
| DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity |
| Ferric ion reducing antioxidant power (FRAP assay) |
| Ferrous chelating activity (FCA assay) |
| Lipid peroxidation inhibition (LPI assay) |
| Phosphomolybdenum (PM) assay |
| Reducing power (RP assay) |
| REACTIVE OXYGEN/NITROGEN SPECIES (ROS/RNS) SCAVENGING ACTIVITY |
| Hydrogen peroxide (H2O2) scavenging activity |
| Hydroxyl radical (OH•) scavenging activity |
| Nitric oxide (NO) scavenging activity |
| Superoxide radicals (O2•–) scavenging activity |
| OXIDATIVE STRESS MARKER CONTENTS |
| Hydrogen peroxide (H2O2) production |
| Superoxide radical (O2•-)production |
| Malondialdehyde(MDA) content |
BIOLOGICAL ACTIVITY OF COMMERCIAL
NATURAL PLANT PRODUCTS
| SKINCARE AND ANTI-AGING PRODUCTS |
| UV-protective activity (UV-spectra method) |
| Skin Depigmentation (Inhibition of Tyrosinase activity) |
| Anti-aging and anti-wrinkle activity (Inhibition of Elastase activity) |
| Anti-aging and anti-wrinkle activity (Inhibition of Collagenase activity) |
| ANTIDEPRESSANT PRODUCTS |
| Antidepressant activity (Inhibition of Monoamine oxidase A activity) |
| NEUROPROTECTIVE PRODUCTS |
| Neuroprotective activity (Inhibition of Acetylcholinesterase activity) |
| Neuroprotective activity (Inhibition of Butyrylcholinesterase activity) |
| ANTI-INFLAMMATORY PRODUCTS |
| Anti-inflammatory activity (Inhibition of β-glucuronidase activity) |
| Anti-inflammatory activity (Inhibition of Lipoxygenase activity) |
| Anti-inflammatory activity (Inhibition of Xanthine oxidase activity) |
| ANTIDIABETIC PRODUCTS |
| Antidiabetic activity (Inhibition of α-amylase activity) |
| Antidiabetic activity (Inhibition of α-glucosidase activity) |
| ANTIHYPERLIPIDEMIC PRODUCTS |
| Antihyperlipidemic activity (Inhibition of Pancreatic lipase activity) |
| Antihyperlipidemic activity (Inhibition of Cholesterol esterase activity) |
| GASTROPROTECTIVE PRODUCTS |
| Gastroprotective activity (Inhibition of Urease activity) |
| CARDIOPROTECTIVE PRODUCTS |
| Cardioprotective activity (Inhibition of Phosphodiesterase activity) |
| Antitoxic Products |
| Antitoxic activity (Inhibition of Carboxyl esterase activity) |
| ANTITOXIC PRODUCTS |
| Antiprotease activity (Inhibition of α-chymotrypsin) |
| Antiprotease activity (Inhibition of trypsin) |
| IN VITRO DIGESTION OF NATURAL PRODUCTS (BIOACCESSIBILITY AND BIOAVAILABILITY) |
| In vitro oral digestion (Simulation of salivary digestion) |
| In vitro gastric digestion (Simulation of gastric digestion) |
| In vitro intestinal digestion (Simulation of intestinal digestion) |
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