PLANT TISSUE CULTURE AND ANALYITICAL SPECIALISTS
Micropropagation

Plant in vitro technology is applied for vegetative reproduction (propagation) allowing large-scale and year-round production of plants for short time period (about 90 days).

  • Selection of donor-plant

    The plant with elite characteristics could be selected by the customer.

  • Isolation of primary explants

    The primary explants (buds, leaves, stems or roots) for propagation could be isolated either from field-grown plants that undergo to process of surface sterilization or directly from aseptically germinated seeds.

  • Inoculation of explant

    The explants are inoculated on nutrient medium supplemented with phytohormones (cytokinins/auxins) to induce growth and development of new plant shoots. In vitro plants are maintained in growth chambers with well-controlled temperature, light and humidity. This phase of new plants induction takes 30-40 days.

  • Multiplication of explants

    The multiplication of plant shoots is performed by their subcultivation on fresh medium with high concentration of cytokinins to obtain indefinite number of lateral shoots (axillary branching). In optimal in vitro conditions, plant multiplication takes about 30 days.

  • Rooting of multiplied shoots and regeneration of whole plantlets

    Multiplied plant shoots are subcultured on а rooting medium with auxins for regeneration of whole in vitro plants. The rooting (rhizogenesis) of plants is achieved for about 30 days.

  • Acclimatization

    The acclimatization of in vitro plants is а key step of their successful adaptation to ex vitro conditions. In vitro plants are transferred into sterile mixture of send, perlite and peat (1:1:1) and kept under controlled conditions in culture room or green house (10-14 days).

  • Adaptation

    The acclimatized plants are then transferred to outdoor conditions.

This Company uses in vitro technology for commercial plant production as well as phytochemical screening and bioactivity testing


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